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Micro- and small enterprises are of great importance for the economic growth in developing countries, as they contribute to employment creation and innovation. In light of their economic relevance, several approaches to support micro- and small enterprises have emerged, including building human capital through business trainings. However, the effects of existing business trainings on entrepreneurial success have so far been limited. One promising alternative training approach that has emerged in the last years is personal initiative training, which teaches self-starting, future-oriented, and persistent entrepreneurial behavior. This dissertation helps to improve the understanding of personal initiative training by shedding light on the mechanisms through which it affects business success, on supporting factors, and on its long-term impacts. Chapter 1 provides an overview on the topic of personal initiative training for entrepreneurs in developing countries. Chapter 2 introduces personal initiative training and other proactive behavior trainings in various contexts of work, including entrepreneurship. The chapter presents action regulation theory and the theory on personal initiative as the theoretical foundation of the training. In addition, the chapter provides insights into training and evaluation methods and makes recommendations for the successful implementation of personal initiative training. Chapter 3 offers a first answer to the question how personal initiative after training can be maintained over time. The chapter introduces training participants' need for cognition as beneficial factor for post-training personal initiative maintenance. Chapter 4 explains how action regulation trainings like personal initiative training contribute to poverty reduction in developing countries by supporting entrepreneurial success. Chapter 5 enlarges upon the topic of personal initiative training for entrepreneurial success in developing countries. The chapter focuses on how personal initiative training supports female entrepreneurs in developing countries by helping them to overcome the uncertainty involved in entrepreneurial actions. Chapter 6 summarizes the overall findings and illustrates the theoretical and practical implications that result from this dissertation. In sum, this dissertation makes a contribution to the better understanding of personal initiative training and its effects on entrepreneurship in developing countries and thereby helps to create effective interventions to combat poverty in developing countries.