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A central aspect of sustainability governance is collaboration, which has been lauded for its benefits but also criticized for its challenges. The potential benefits of collaboration have apparently been recognized also in the context of EU agriculture. Yet, there has been a lack of holistic consideration of how collaboration can be systematically integrated and promoted in the governance of EU agriculture. Sustainable agriculture cannot only be encouraged through changes in the overall governance system but also through the support of existing and emerging small-scale collaborative initiatives for sustainable agriculture. Indeed, there has been substantial research on the conditions that influence success of similar collaborative initiatives. However, the knowledge resulting from this research remains rather scattered and does not allow for the identification of overall patterns. Additionally, little of this research specifically focuses on sustainable agriculture. What is more, the promotion of collaboration for sustainable agriculture is further complicated by the lack of clarity of the meaning of sustainable agriculture, which is an inherently ambiguous and contested concept. This cumulative dissertation aims to address these gaps by contributing to a better understanding of how collaboration can be facilitated and designed as a means to govern for and advance sustainable agriculture. For this purpose, the dissertation addresses three sub-aims: 1) Advancing the understanding of the concept of sustainable agriculture; 2) scrutinizing the current governance system regarding its potential to facilitate or hamper collaboration; 3) assessing conceptually and empirically how actor collaboration can be facilitated as a means to govern for sustainable agriculture, both from a top-down and a bottom-up perspective. In doing so, this dissertation focuses on EU agriculture and applies a mix of methods, ranging from qualitative to quantitative dominant. The findings of this dissertation highlight that collaboration has been underappreciated and even hampered as an approach to governing for sustainable agriculture. In contrast, this dissertation argues that collaboration offers one promising way to promoting and realizing agriculture and emphasizes the need to integrate different approaches to collaboration and to sustainable agriculture.