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The emergence of sustainability as a guiding principle for tourism development came along with needs to introduce instruments that can monitor the actual impacts of tourism. Sustainability assessments in tourism (SAT) have gained popularity in recent years with a range of measurement schemes being introduced for national and subnational tourism destinations. With the help of sustainability indicators these schemes intend to guide decision-makers in making better evidence-informed decisions and to improve the overall sustainability performance of tourism. With this dissertation, the author aims to contribute to a deeper understanding of the implementation and performance of sustainability assessments, by linking transformative needs of tourism with necessary assessment approaches that can serve as effective instruments for a shift towards a more sustainable tourism development. Thus, the research is part of recent efforts to establish profound and effective measurement approaches for sustainable tourism. The author employs a mixed-methods approach combining qualitative, quantitative, set-theoretic, and review methods, with the aim of maximising the validity of results. First, he explores the general progress and current state of research on sustainability assessments in tourism, with the intention to identify patterns, key elements and research gaps within assessment approaches This is followed by subsequent detailed analyses that examine specific environmental and socio-economic sustainability issues with the aim of providing conceptual, methodological and empirical solutions for assessing them in detail. The dissertation highlights that concrete assessment tools are needed for evidence-informed decision-making and the establishment of effective actions in destination management. The findings indicate that assessments will be more successful in terms of serving as tools for decision-making, if they tackle main drivers of change and encourage management or policymakers to take decisions that affect multiple sustainability issues. It also reviews different concepts and accounting principles and rises awareness of a cautious selection of methods and measurement approaches, as this may affect overall results. The thesis empirically evaluates and applies different measurement approaches in specific destinations, with the help of quantitative and qualitative data collection methodologies. In general, my thesis provides further clarification about key environmental and socio-economic measurement methodologies, which supports ongoing debates about sustainability impacts of tourism. Thus, the research contributes to knowledge, frameworks, methodologies and practical application for tourism governance and tourism sustainability science.